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Paper - I
General Microbiology, Immunology, Sytematic bacteriology, Microbiological techniques

Unit - I : General Microbiology

1. History of Microbiology - Important contributions and Contributers.
2. General Properties of Bacteria.
   1. Morphology and Cell Structure.
   2. Metabolism, pathways and enzymes.
   3. Growth and cultivation - Growth curve, Nutrients and Media
3. Control of Microorganisms
   1. Sterilisation
   2. Disinfectants
   3. Anti Microbial agents
4. Genetics
5. Host parasite relationship - Normal flora, pathogens, Routes of infection, virulence factors.
6. Use and Care of laboratory animals
7. Principles of epidemiology of infections

Unit - II : Immunology

1. Structure and development of Immune system
2. Cells involved in Immune systems
3. Non specific resistance mechanisms
4. Antigens
5. Immunoglobulins - structure and function
6. Complement system
7. Antigen, Antibody reactions and applications
8. Specific immune response, humoral and cellular against micro organisms
9. Hypersensitivity reactions (Different types)
10. Major histocompatibility complex - HLA systems
11. Transplantation immunity
12. Autoimmunity (Mechanism and diagnosis)
13. Immuno deficiency mechanisms and diagnosis
14. Principles of Tumour immunology
15. Immunisation and Immunotherapy

Unit - III : Systematic Bacteriology

Morphology, cultural characteristics, pathogenicity, laboratory diagnosis, and principles of management and control and epidemiology of the following medically important bacteria.

1. Gram positive cocci - Staphylococci, streptococci, Entrococci
2. Gram Negative Cocci - Gonococci, Meningococci, Brahamella
3. Gram positive non sporing bacilli - Corynebacteria, Actinomycetes (aerobic and anaerobic)
4. Gram positive spore forming bacilli - Bacillus, Clostridia
5. Acid fast bacteria - M. tuberculosis, M.leprae, other mycobacteria
6. Small gram negative bacteria - Haemophilus, Bordetella, Yersinia, Brucella
7. Enteric Gram Negative bacilli
   1. Entcrobacteriaceae
   2. Vibrios
   3. Campy labacter
   4. Helicobacter
8. Pseudomonas and other related non fermenting organisms.
9. Legionella
10. Anaerobic gram negative bacilli
    1. Bacteriodes
    2. Prevotella
    3. Porphyromonas
    4. Fusobacterium
11. Spirochaetes : treponema, leptospira, borellia
12. Mycoplasma
13. Chlamydia
14. Rickettsiae

Unit - IV : Microbiological Techniques

1. Microscopy -
   1. Different types and usefullness
   2. Technical specifications
   3. General maintenance
2. Staining - Preparation and use of stains for bacteriology, parasitology,mycology and virology
3. Specimen collection, preservation, transport, processing
4. Principles and techniques employed in indentification of micro organisms.
5. Immunisation
   1. Hyperimmune sera preparation
   2. Use of adjuvants
6. Maintenance of stock cultures
7. Blood grouping, Rh typing, cross matching.
8. HLA typing
9. Principle and techniques of sera diaganosis of infections
10. Maintenance of equipments - Deep freezers, centrifuges etc.
11. Methods of assessing status of immune system.
    1. Complement system
    2. Estimation of T & B Cells
    3. Estimation of antibodies
    4. Tests for hypersensitivity reactions
    5. Tests for auto immune diseases
12. Preparation and uses of monoclonal antibodies.

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Paper - II

Virology, Parasitology, Mycology, Laboratory, Management, Applied Microbiology and Recent advances

Unit - I : Virology

1. General Properties of all RNA and DNA virus families of medical importance and prions
2. Pathogenesis and pathology of individual viral diseases
3. Laboratory diagnosis (including molecular techniques) of viral diseases
4. Antiviral therapy
5. Viral vaccines and antisera
6. Epidemiology and control of viral diseases
7. Oncogenis viruses
8. Bacteriophages
9. Tissue culture techniques

Unit - II : Parasitology

Morphology, life cycle, pathogenesis, laboratory diagnostic methods,drug used for therapy, and epidemiology of following parasites :-

1. Protozoa :-
   1. Intestinal amoebae
   2. Free living pathogenic amoebae
   3. Intestinal and genital flagellates
   4. Haemoflagellates
   5. Ciliates of medical importance
   6. Malarial parasites
   7. Opportunistic protozoa - P - carinii, Toxoplasma, Cryptosporidium, microsporidia



2. Helminths
   1. Nematodes -
      a) Intestinal
      b) Tissue
   2. Cestodes
   3. Trematodes
   4. Larva migrans



3. Entomology : related to transmission of parasitic infections

Unit - III : Mycology

1. General properties of Yeast like fungi, filamentous fungi, dimorphic fungi
2. Isolation and Identificaiton of medically important fungi
3. Morphology, cultural characteristics, pathogenesis, laboratory diagonis, treatment and epidemiology of the following :-
   1. Candida and Cryptococci
   2. Dermatophytes
   3. Dimorphic fungi
   4. Agents of mycetoma
   5. Agents of subcutaneous mycosis
   6. Opportunistic fungi.
4. Mycotoxins

Unit - IV : Laboratory Management

1. Quality control in Microbiology
   1. External
   2. Internal
2. Safety in Microbiology laboratory
3. Staff - Requirements, pattern, training, Continuing education.
4. Materials purchase, storage, accounting maintenance equipment - glassware, reagents and chemicals.
5. Laboratory design and maintenance
6. Records - Requisitions, reporting, recording, including computerisation, statistics.
7. Budget and costing
8. Research design and methodology

Unit - V : Applied Microbiology (Clinical, Industrial, food)

1. Nosocomial infections
   1. Types
   2. Surveillence
   3. Investigation of an outbreak
   4. control
2. Etiology and laboratory diagnosis of infections of each system
   1. Urinary tract
   2. respiratory tract
   3. Gastro intestinal Tract
   4. Central nervous system
   5. Cardio vascular system and blood
   6. Sexually transmitted diseases
   7. Genital tract
3. Infections in immuncompromised host
4. Microbiology of air, water, milk and food
5. National programmes in prevention of infectious diseases.

Unit - VI : Recent Advances

1. Emerging and Reemerging infections
2. Laboratory techniques
3. Bacteriology
4. Virology
5. Immunology
6. Parasitology
7. Mycology