Tamil Nadu Public Service Commission Exam Syllabus - Biotechnology

Paper - I
(Basic Sciences)


Unit - I : Microbiology
Microbiology classification, monophology, characterisation and isolation
Metabolism, Growth
Environmental effect
Axenic mixed culture concept - interaction and ecosystem model.

Unit - II : Immunology And Virology
Immunity and antibodies,
Immuno regulations
Molecular variability of antibody specificity
Immunological techniques - immuno electrophoresis - etc.,
Ultrastructure of viruses,
DNA and RNA viruses
Viral infections
Retroviruses
Interferons - mechanism of action etc.

Unit - III : Cell And Tissue Culture
Different cell lines - characterisation and maintenance
Hybridoma technology
Tissue organisation
Mechanical factors analysis.

Unit - IV : Genetics And Molecular Biology
Chromosome, Plasmids
Genetic mapping
Mandelian and Non-Mandelian Principles
Genetics and Society
DNA and RNA
Coding system - genetic regulation
Operon - promoter - repressor relation
Eucaryotic regulation
Unit - V : Applied Genetics
R-DNA Protoplast Fusion
Role of d NTPs
In vitro DNA Synthesis
Site - directed mutagenesis - PCR, SSDNA and DSDNA Cloning
Vectors - Properties etc., Restriction mapping
c DNA and Genomic library
RFLP

Unit - VI : Physiology
Special Sensery mechanisms
Body fluids - Properties etc.,
Histological studies of organs and tissues

Unit - VII : Biochemistry And Biophysics

• Supra molecular assemblies
• Biomembrane
• Metabolism
• Bioenergetis
• Biopolymer structure and function
• Irreversible thermodynamics in biology

Unit - VIII : Bio-Inorganic & Bio-Organic Chemistry
Metalloproteins
Transition metal in biological reactions
Role of chelating agents and metals in medicine
Trace elements absorbtion
Transport and tissue storage
Carbohydrate biopolymers
Glycoproteins

Unit - IX : Membrane Science
Analysis of membrane assymetry
Lipid - protein interactions
Lipid components in membranes
Lipsomes
Membrane potentials
Membrane bound enzymes, transport and receptors

Unit - X : Bio Analytical Techniques
IR, NMR, Mass spectrometry principles
Ion beam technique
Time resolved fluorescenes spectroscopy
X-ray crystallography
Microscopy

Paper - II
(Applied & Engineering Subjects)


Unit - I : Bio-Process Technology
Energy, Mass and momentum transfer
Material and enrgy balances
Upstreaming operations - Sterilization, air filter design
Biological process principle
Statistical optimisation techniques in biological system - Plackett - Burman, CCD, ANOVA analysis and contour interpretation.
Cellualar and enzymatic reactions.

Unit - II : Protein Engineering And Bio-Informatics
Protein data bank
Protein structures; structure & function analysis
Domain analysis in proteins
Protein design, protein sequencing

Unit - III : Bio-Conversion And Fermentation Technology
Bioconversion of starch, cellulose, corn
Bioenrgy production
Industrial applications of cells and enzymes
Biopolymer design and synthesis
Fermentation technologies for waste stabilization and bio-product synthesis.
Antibiotics and steroid fermentation

Unit - IV : Downstream Technology
Flocculation, Coagulation, Centrifugation
Equilibrium (stage) processes - distillation and liquid - liquid extraction.
Chromatography - GPC, Ion-exchange, affinity
Separation of intracellular products
Membrane Separations - Reverse Osmosis, ultrafiltration, Microfiltration
Aqueous two phase system
Supercritical fluid extraction.

Unit - V : Kinetics And Thermodynamics In Bio-Processes
Moinod's equation - generalized Monod' equaiton
Structured models
Application of Arrhenius equation
Stability analysis of cellular and enzymati porcesses - D*, DS*, DH* calculation.

Unit - VI : Bio-Reactors
Basic principles of bioreactors
Configuration and their analysis
Non-ideal effects
Control of bioreactor
Bioreactor modelling and stability analysis
Mechanical design concept

Unit - VII : Process Design And Scale-Up
Flow sheet analysis
Identification of crucial factors in processes
Computer - aided design
Scale-up criteria - k,a, power number etc.
Economic analysis for process scale-up and scale-down

Unit - VIII : Food Technology
Components in food
Basal metabolic rate
Long term preservation - canning, freeze drying, sprary drying
UHT concept and pasteurization

Unit - IX : Pharmaceutical Science And Engineering
Pharmacekinetics
Major unit operations in pharmaceutical processing of antibiotics., life saving drugs and their formulation.
Concept of LD50.

Unit - X : Laboratory Hygiene And Environment Safety
Biosafety regulations
Handling of genetically enginured organisms
Disposal of radioactive components and otehr health hazard substances
Public health and pollution problems
Waste management, recycling