TNPSC Bio-Chemistry Syllabus

Bio-Chemistry (M.D) - (Code - 017)

Paper I
Paper II


Paper - I


Unit - I : Cells and Cellular Components
Organisation and Components of excaryatic cells
Functional role of sub cellular organelles.
Methods of Separation of Sub cellular organelles

Unit - II : Composition, Structure and function of Biomolecules

Carbohydrates Monosaccharides,disaccharides,Polysaccharides,Fibres, Amino sugars and muco polysaccharides.
Lipids Fatty acid, Triacylglycerol, Phospholipids, Cholesterol, Lipoproteins, essential Fattyacids and their derivatives.

Unit - III : Enzymology
Classification, Mechanism of action, enzyme kinetics, factors regulating enzyme action
Regulation of enzyme action, enzyme inhibitors
Enzymes of clinical importance
Isoenzymes, Co-enzymes and Co-factors (metallo enzymes)

Unit - IV : Biological Oxidation
Enzymes of oxidation, Components of Electron transport chain
Oxidative Phosphorylation
Inhibitors of Electron transport chain

Unit - V : Digestion and absorption of Nutrients Carbohydrates, Proteins and Lipids

Unit - VI : Nutrition Principles involved - BMR,SDA,RDA etc - Protein energy metabolism & Nutritional disorders

Unit - VII : Vitamins Fat soluble Vitamins and its Deficiency disorders Water soluable Vitamins,its co-enzyme functions and Deficiency disorders Vitamins assay.

Unit - VIII : Metabolism and Regulation
Carbohydrate -Glycolysis, TCACycle, HMPshuntUronic acidpathway, Galactose Glycogenmetabolism, Gluconeogenesis, Metabolism of fructose
Diabetes Mellitus-Glycogen Storage Disorders- essential Fructosuria - Fructose intolerance-galactosemia-Lactose intolerance
Lipids-Fattyacid Synthesis and oxidation - Synthesis of TAG, Phospholipids Metabolism of Cholesterol, Lipo Proteins and Glycolipids
Lipid Storage disorders,Refsum Disease,Lipoproteinemia

Paper - II

Unit - I : Strucutre and Functions of Proteins and Nucleic AcidAmino Acids, Structure of Protein, Biolgically important Peptides, Phsiological Proteins like Hemoglobin, Structural Protein, Myoglobin, Collagen, Immuno globulins, Glycoprotein and Plasma Proteins. Nucleic Acids-DNA and RNA-Nucleotide Analogues

Unit - II : Metabolism of Amino Acid - Amino Acid Nitrogen and Urea Synthesis. Fate of Carbon Skeleton of Individual Amino AcidsintoSpecializedProducts - Hemoglobin synthesis and breakdown, bilirubin metabolism - Nucleic acids - Purine and pyramidine - synthesis and breakdown

Unit - III : Disorders associated with regulatory pathwayInborn errors of metabolism - Proteins - Inborn errors of metabolism associated with individual amino acids - Urea cycle disorders- Nucleic acids-Gout,Lesch-Nyhan syndrome, orotic aciduria and other inherited disorders - Porphyrias -Hemoglobinopathies and Hyper bilirubinaemias

Unit - IV : Molecular Biology Genetic code,Replication,Transcription,translation,post translational modification -Regulation of gene expression. - Mutation and repair of DNA damage. DNA Recombinant Technology - its application,gene therapy and molecular basis of diseases

Unit - V : Water and electrolytes and its associated disorders.

Unit - VI : Acid base balance and its disorders Principles involved - BMR, SDA, RDA etc - Protein energy metabolism & Nutritional disorders

Unit - VII : Hormones and their related disorders Mechanism of action - Peptide hormones and Steroid hormones etc

Unit - VIII : Functional testsAssesment of functions of various organs such as Liver, Kidney, Thyroid,Pancreas,GIT,Parathyroid, adrenals and Pituitary

Unit - IX : Priciples of conventional and spcialized laborotory investigations and Instrumentation

Unit - X : Investigations and Interpretations of results in Health and Disease